A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils.

A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils.

Heliyon. 2019 Jul;5(7):e02048

Authors: Sano M, Kuroda H, Ohara H, Ando H, Matsumoto K, Aso Y

Abstract
In this study, we report a novel method based on the Mizoroki-Heck reaction followed by an iodine test for the screening of itaconic acid-producing fungi from soils. This method is simple, rapid, and requires 10 μL of culture; results are obtained within 1.5 h. The detection limit of itaconic acid in the cultures was 0.13 mM. This is the first report on the direct screening of itaconic acid-producing fungi using a coupling reaction.

PMID: 31372531 [PubMed]

Source: Industry