Cloning and Expression of Heterologous Cellulases and Enzymes in Aspergillus niger.
Methods Mol Biol. 2018;1796:123-133
Authors: Reilly MC, Campen SA, Simmons BA, Baker SE, Gladden JM, Magnuson JK
Cellulases and other enzymes are needed for saccharification of plant biomass in the biorefinery industry. Expression, characterization, and eventual large-scale production of known and novel cellulases requires the ability to express and secrete heterologous enzymes in relevant protein production platforms like Aspergillus niger. A method for cloning and expression of genes for these desirable enzymes in A. niger is presented in this Chapter.
PMID: 29856051 [PubMed – in process]