Culture Cell Block Controls as a Tool to the Biomolecular Diagnosis of Infectious Diseases.
Appl Immunohistochem Mol Morphol. 2019 Oct 17;:
Authors: José Tadeu de Araujo L, Salas-Gómez D, Midori Kimura L, Fernandes Possatto Takahashi J, de Souza Barrel J, Rollin DC, Mariotti Guerra J
The cell block (CB) technique has allowed easy obtainment of samples such as cellular and culture suspensions, to perform specific molecular tests such as immunohistochemistry and in situ hybridization. It has been improved along time, accuracy, and quality of the diagnoses, however, the cost of a commercial gel matrix for the preparation of CB is high and not suitable depending on the situation. The objective of this study is to test agarose as an alternative to the commercial gel matrix in the preparation of Aspergillus fumigatus’ CB.
PMID: 31633490 [PubMed – as supplied by publisher]