Diagnostic Performance of Immunohistochemistry for the Aspergillosis and Mucormycosis.
Mycoses. 2019 Aug 24;:
Authors: Choi S, Song JS, Kim JY, Cha HH, Yun JH, Park JW, Jung KH, Jo KM, Jung J, Kim MJ, Chong YP, Park YS, Lee SO, Choi SH, Kim YS, Woo JH, Kim SH
OBJECTIVE: To investigate the accuracy of immunohistochemistry (IHC) tests for distinguishing between mucormycosis and aspergillosis and compare the clinical characteristics of mucormycosis patients according to galactomannan (GM) results.
METHODS: We evaluated diagnostic performance of IHC test with tissue sections of patients with culture-proven invasive fungal infection. In addition, we conducted PCR assay with tissue sections of mucormycosis patients with positive GM results to evaluate the possibility of co-infection.
RESULTS: In culture-proven mucormycosis (n = 13) and aspergillosis (n = 20), the sensitivity and specificity of IHC test were both 100% for mucormycosis and 85% and 100%, respectively, for aspergillosis. Among the 53 patients who met the modified criteria for proven mucormycosis and had GM assay results, 24 (45%) were positive. Compared with those with negative GM results (n = 29), mucormycosis patients with positive GM results had significantly higher incidence of gastrointestinal tract infections (6/24 [25%] vs. 0/29 [0%], P = 0.006), and were more likely to be histomorphologically diagnosed as aspergillosis (7/24 [29%] vs. 2/29 [7%], P = 0.06). PCR assay amplified both Aspergillus- and Mucorales-specific DNA in 6 of these 24 cases.
CONCLUSIONS: IHC tests seem useful for compensating for the limitations of histomorphologic diagnosis in distinguishing between mucormycosis and aspergillosis. Some proven mucormycosis patients with positive GM results had histopathology consistent with aspergillosis and gastrointestinal mucormycosis. In addition, about one quarter of these patients revealed the evidence of co-infection with aspergillosis by PCR assay. This article is protected by copyright. All rights reserved.
PMID: 31444927 [PubMed – as supplied by publisher]