Functional Characterization of a Cystathionine β-Synthase Gene in Sulfur Metabolism and Pathogenicity of Aspergillus niger in Pear Fruit.

Functional Characterization of a Cystathionine β-Synthase Gene in Sulfur Metabolism and Pathogenicity of Aspergillus niger in Pear Fruit.

J Agric Food Chem. 2019 Apr 04;:

Authors: Guo SX, Yao GF, Ye HR, Tang J, Huang ZQ, Yang F, Li YH, Han Z, Hu LY, Zhang H, Hu K

Abstract
Aspergillus niger, which is a fungal pathogen, causes rot in a variety of fruits. In this study, the cystathionine β-synthase cbsA gene was deleted by homologous recombination to study its role in sulfur metabolism and the pathogenicity of A. niger. The results showed that ΔcbsA strain maintained normal mycelia growth and sporulation compared with the control strain A. niger MA 70.15, whereas the contents of cysteine and glutathione (GSH) increased significantly after cbsA deletion. However, ΔcbsA strain showed reduced endogenous H2S production. Further results showed that cbsA gene deletion induced higher resistance to cadmium stress and stronger infectivity to pears. It was also found that a stronger response of reactive oxygen species (ROS) production was induced in ΔcbsA mutant-infected pear compared with the control strain. In all, the present research suggested the important role of cbsA in sulfur metabolism and pathogenicity of A. niger on pear fruit.

PMID: 30945533 [PubMed – as supplied by publisher]

Source: Industry