Genetic and functional analysis of the DKxanthene biosynthetic gene cluster from Myxococcus stipitatus DSM 14675.

Genetic and functional analysis of the DKxanthene biosynthetic gene cluster from Myxococcus stipitatus DSM 14675.

J Microbiol Biotechnol. 2018 Jun 08;:

Authors: Hyun H, Lee S, Lee JS, Cho K

Abstract
DKxanthenes area class of yellow secondary metabolites produced by myxobacterial genera Myxococcus and Stigmatella. We identified a putative 49.5 kb DKxanthene biosynthetic gene cluster from M. stipitatus DSM 14675 by genomic sequence and mutational analysis. The cluster was comprisedof 15 genes (MYSTI_06004-MYSTI_06018) encoding polyketide synthases, non-ribosomal peptide synthases, and proteins with unknown functions. Disruption of the genes by plasmid insertion resulted in defects in the production of yellow pigments. High-performance liquid chromatography and liquid chromatography-tandem mass spectrometry analysis indicated that the yellow pigments produced by M. stipitatus DSM 14675 might be noble DKxanthene derivatives.M. stipitatus did not require DKxanthenes for the formation of heat-resistant viable spores,unlike M. xanthus. Furthermore, DKxanthenes showed growth inhibitory activity against the fungi Aspergillus niger, Candida albicans, and Rhizopus stolonifer.

PMID: 29975996 [PubMed – as supplied by publisher]

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