Microbial transformation ethisterone by Aspergillus niger and Cunninghamella blakesleeana.

Microbial transformation ethisterone by Aspergillus niger and Cunninghamella blakesleeana.

Steroids. 2019 Aug 07;:108467

Authors: Aziz A, Bano S, Atia-Tul-Wahab, Iqbal Choudhary M

Abstract
Ethisterone (17α-ethynyl-17β-hydroxyandrost-4-en-3-one) (1) is a synthetic steroidal estrogen. It is extensively used as an oral contraceptive. The current study involves biotransformation of ethisterone (1) by Aspergillus niger, and Cunninghamella blakesleeana. Fermentation of 1 with C. blakesleeana afforded two new polar metabolites 17α-ethynyl-6β,15β,17β-trihydroxyandrost-4-en-3-one, 17α-ethynyl-7β,15β,17β-trihydroxyandrost-4-en-3-one, while transformation of ethisterone with A. niger yielded a new metabolite 17α-ethynyl-6α,17β-dihydroxyandrost-4-en-3-one, along with a known metabolites, 17α-ethynyl-11α,17β-dihydroxyandrost-4-en-3-one. Modern spectroscopic techniques were used to characterize the structures of all transformed products.

PMID: 31400394 [PubMed – as supplied by publisher]

Source: Industry