Molecular identification, antifungal susceptibility testing and mechanisms of azole resistance in Aspergillus spp. received within a surveillance program on antifungal resistance in Spain.

Molecular identification, antifungal susceptibility testing and mechanisms of azole resistance in Aspergillus spp. received within a surveillance program on antifungal resistance in Spain.

Antimicrob Agents Chemother. 2019 Jul 08;:

Authors: Rivero-Menendez O, Soto-Debran JC, Medina N, Lucio J, Mellado E, Alastruey-Izquierdo A

Abstract
Antifungal resistance is one of the major causes of the increasing mortality rates in fungal infections, especially for those caused by Aspergillus spp. A surveillance program was established in 2014 in the Spanish National Center for Microbiology for tracking resistance on the most prevalent Aspergillus species. 273 samples were included in the study and were initially classified as susceptible or resistant according to EUCAST breakpoints. Several Aspergillus cryptic species were found within the molecularly identified isolates. Cyp51 mutations were characterized for A. fumigatus, A. terreus and A. flavus sensu stricto (s.s.) strains that were classified as resistant three A. fumigatus s.s. strains carried the TR34/L98H resistance mechanism, while two harbored G54R substitution and one the TR46/Y121F/T289A mechanism. Seventeen strains had no mutations in cyp51A, being ten of them resistant only to isavuconazole. Three A. terreus s.s. strains harbored D344N substitution in cyp51A, one of them combined with M217I, and another carried A249G novel mutation. A. flavus s.s. itraconazole resistant strains harbored P220L and H349R alterations in cyp51A and cyp51C, respectively, that need further investigation on their implication in azole resistance.

PMID: 31285229 [PubMed – as supplied by publisher]

Source: Industry