Respiratory specimens and the diagnostic accuracy of aspergillus lateral flow assays (LFA-IMMYTM): real life data from a multicenter study.

Respiratory specimens and the diagnostic accuracy of aspergillus lateral flow assays (LFA-IMMYTM): real life data from a multicenter study.

Clin Microbiol Infect. 2019 Aug 21;:

Authors: Lass-Flörl C, Lo Cascio G, Nucci M, Camargo Dos Santos M, Colombo AL, Vossen M, Willinger B

Abstract
OBJECTIVES: Proper diagnosis of invasive aspergillosis is challenging as conventional methods lack sensitivity and are complicated due to time-consuming incubation processes. In order to meet the requirement for early diagnosis the new Aspergillus specific point-of-care test LFA-IMMYTM was evaluated with respect to the ability to accurately detect Aspergillus in broncho-alveolar fluids and sputa and to clarify the potential of cross reactivity with other fungal pathogens.
METHODS: Respiratory specimens (n=398) from non-selected patients (n=390) underwent either fungal microscopy, culture or both prior to Aspergillus lateral flow assay (LFA-IMMYTM) testing.
RESULTS: For Aspergillus culture and microscopy positive samples, sensitivity (48/52) and specificity (44/48) was 92% (95%CI 8.0-9.7) and 91% (95%CI 7.9-9.7), respectively; cross-reactivity was documented with non-Aspergillus pathogens.
CONCLUSION: LFA-IMMYTM is a reliable diagnostic tool for the detection of Aspergillus being present in respiratory samples.

PMID: 31445208 [PubMed – as supplied by publisher]

Source: Industry