The HosA histone deacetylase regulates aflatoxin biosynthesis through direct regulation of aflatoxin cluster genes.

The HosA histone deacetylase regulates aflatoxin biosynthesis through direct regulation of aflatoxin cluster genes.

Mol Plant Microbe Interact. 2019 Apr 15;:

Authors: Lan H, Wu L, Sun R, Keller NP, Yang K, Ye L, He S, Zhang F, Wang S

Abstract
Histone deacetylases (HDACs) always function as co-repressors and sometimes as co-activators in the regulation of fungal development and secondary metabolite production. However, the mechanism through which HDACs play positive roles in secondary metabolite production is still unknown. Here, classical HDAC enzymes were identified and analyzed in Aspergillus flavus, a fungus that produces one of the most carcinogenic secondary metabolites, aflatoxin B1 (AFB1). Characterization of the HDACs revealed that a class I family HDAC, HosA, played crucial roles in growth, reproduction, the oxidative stress response, AFB1 biosynthesis, and pathogenicity. To a lesser extent, a class II family HDAC, HdaA, was also involved in sclerotia formation and AFB1 biosynthesis. An in vitro analysis of HosA revealed that its HDAC activity was considerably diminished at nanomolar concentrations of Trichostatin A. Notably, chromatin immunoprecipitation experiments indicated that HosA bound directly to AFB1 biosynthesis cluster genes to regulate their expression. Finally, we found a transcriptional regulator SinA, interacts with HosA, to regulate fungal development and AFB1 biosynthesis. Overall, our results reveal a novel mechanism by which classical HDACs mediate the induction of secondary metabolite genes in fungi.

PMID: 30986121 [PubMed – as supplied by publisher]

Source: Industry