The PDR-type ABC transporters AtrA and AtrG are involved in azole drug resistance in Aspergillus oryzae.

The PDR-type ABC transporters AtrA and AtrG are involved in azole drug resistance in Aspergillus oryzae.

Biosci Biotechnol Biochem. 2018 Jul 16;:1-9

Authors: Miura D, Sugiyama K, Ito A, Ohba-Tanaka A, Tanaka M, Shintani T, Gomi K

Abstract
For strain improvement of Aspergillus oryzae, development of the transformation system is essential, wherein dominant selectable markers, including drug-resistant genes, are available. However, A. oryzae generally has a relatively high resistance to many antifungal drugs effective against yeasts and other filamentous fungi. In the course of the study, while investigating azole drug resistance in A. oryzae, we isolated a spontaneous mutant that exhibited high resistance to azole fungicides and found that pleiotropic drug resistance (PDR)-type ATP-binding cassette (ABC) transporter genes were upregulated in the mutant; their overexpression in the wild-type strain increased azole drug resistance. While deletion of the gene designated atrG resulted in increased azole susceptibility, double deletion of atrG and another gene (atrA) resulted in further azole hypersensitivity. Overall, these results indicate that the ABC transporters AtrA and AtrG are involved in azole drug resistance in A. oryzae.

PMID: 30011258 [PubMed – as supplied by publisher]

Source: Industry