Biological investigations of essential oils extracted from three Juniperus species and evaluation of their antimicrobial, antioxidant and cytotoxic activities.

Biological investigations of essential oils extracted from three Juniperus species and evaluation of their antimicrobial, antioxidant and cytotoxic activities.

J Appl Microbiol. 2020 May 23;:

Authors: Elshafie HS, Caputo L, De Martino L, Gruľová D, Zheljazkov VD, De Feo V, Camele I

Abstract
AIMS: To conduct a biological investigations and to evaluate the antimicrobial and antioxidant activities of the essential oil extracted from Juniperus communis, J. scopulorum and J. horizontalis; to screen their mechanisms of action by conducting the cell membrane permeability assay (CMP); and to determine the possible cytotoxicity of the three essential oils against human neuroblastoma cells (SH-SY5Y).
METHODS AND RESULTS: The antifungal activity was tested against four phytopathogenic fungi (Monilinia fructicola, Aspergillus niger, Penicillium expansum and Botrytis cinerea). The antibacterial activity was evaluated against two gram positive (G+ve) (Bacillus megaterium and Clavibacter michiganensis) and three gram negative (G-ve) bacterial strains (Pseudomonas fluorescens, P. syringae pv. phaseolicola and Xanthomonas campestris). Results showed that the three tested EOs have antifungal activity against M. fructicola and P. expansum and effective antibacterial activity against P. syringae pv. phaseolicola and B. megaterium. Moreover, three EOs were evaluated for their ability to inhibit the growth of SH-SY5Y cells with MTT assay. J. communis EO was the more with an IC50 of 53.7μg ml-1 . Our results on antioxidant activity, obtained by DPPH test, showed that there are no significant difference between the tree oils.
CONCLUSIONS: The three tested essential oils showed promising antimicrobial and antioxidant activity and cytotoxic effects against human neuroblastoma cell line.
SIGNIFICANCE AND IMPACT OF STUDY: the outfindings of this research showed promising antimicrobial effects against the majority of the tested phytopathogens with possibility to use plant essential oil as natural alternatives to synthetic drugs, the cause of global environmental problems, pathogen resistance and difficulty to control many post-harvest plant diseases.

PMID: 32445616 [PubMed – as supplied by publisher]

Source: Industry