Vesicle transport and growth dynamics in Aspergillus niger: Microscale modeling of secretory vesicle flow and centerline extraction fromconfocal fluorescent data.

Vesicle transport and growth dynamics in Aspergillus niger: Microscale modeling of secretory vesicle flow and centerline extraction fromconfocal fluorescent data.

Biotechnol Bioeng. 2020 Jun 08;:

Authors: Jakob Kunz P, Barthel L, Meyer V, King R

Abstract
In this paper, we present a mathematical model to describe filamentous fungal growth based on intracellular secretory vesicles (SVs), which transport cell wall components to the hyphal tip. Vesicular transport inside elongating hyphae is modeled as an advection-diffusion-reaction equation with a moving boundary, transformed into fixed coordinates, and discretized using a high-order weighted essentially non-oscillatory discretization scheme. The model describes the production and the consumption of SVs with kinetic functions. Simulations are subsequently compared against distributions of SVs visualized by enhanced green fluorescent protein in young A. niger hyphae after germination. Intensity profile data are obtained using an algorithm scripted in ImageJ that extracts mean intensity distributions from 3D time-lapse confocal measurement data. Simulated length growth is in good agreement with the experimental data. Our simulations further show that a decrease of effective vesicle transport velocity towards the tip can explain the observed tip accumulation of SVs. This article is protected by copyright. All rights reserved.

PMID: 32510171 [PubMed – as supplied by publisher]

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