Efficient fungal and bacterial facilitated remediation of thiencarbazone methyl in the environment.
Environ Res. 2020 Jun 20;188:109811
Authors: Ahmad KS, Gul P, Gul MM
Triazole herbicide, Thiencarbazone-methyl (TCM) applied on different crops for weedicidal activity is associated with an inherent toxicity towards bladder and urinary functionality. TCM has been first time explored for its biodegradative behavior utilizing microbes, previously isolated from soils. Simulated bio-transformation assemblies of five fungal strains; Aspergillus flavus (AF), Penicillium chrysogenum (PC), Aspergillus niger (AN), Aspergillus terrus (AT), Aspergillus fumigatus (AFu) and two bacterial strains: Xanthomonas citri (XC), Pseudomonassyringae (PS), were utilized. 10 mg/L TCM concentration was set up utilizing each microbe and analysed for 42 days. TCM bio-degradation was evaluated by UV-Visible spectrophotometery and gas chromatography mass spectroscopy. Aspergillus terrus (R2 = 0.86) and Penicillium chrysogenum (R2 = 0.88) exhibited highest capability to metabolize TCM while forming intermediate metabolites including; 2,4-dihydro-[1,2,4] triazol-3-one, semicarbazide and urea, methyl 4-isocyanatosulfonyl-5-methylthiophene-3-carboxylate. TCM degradation by all strains AF, AFu, AN, PC, AT, PS and XC was found to be 74, 74, 81, 95, 98, 90 and 95%, respectively after 42 days elucidating the effectiveness of all the utilized strains in degrading TCM. Current investigations can impact vital bioremediation approaches for pesticides mitigation from the ecological compartments. Furthermore, present research can be extended to the optimization of the bio-deteriorative assays to be employed on the practical scale for the successful management of environment through sustainable and cost effective ways.
PMID: 32592941 [PubMed – as supplied by publisher]