Towards a miniaturized culture screening for cellulolytic fungi and their agricultural lignocellulosic degradation.

Towards a miniaturized culture screening for cellulolytic fungi and their agricultural lignocellulosic degradation.

J Microbiol Biotechnol. 2020 Aug 27;:

Authors: Arnthong J, Siamphan C, Chuaseeharonnachai C, Boonyuen N, Suwannarangsee S

Abstract
The substantial use of fungal enzymes to degrade lignocellulosic plant biomass has widely been attributed to the extensive requirement of powerful enzyme-producing fungal strains. In this study, a two-step screening procedure-a miniaturized culture method and shaking-flask fermentation-was proposed and demonstrated for finding cellulolytic fungi. Two hundred ninety-seven fungal strains were isolated from several cellulose-containing samples found in two different locations in Thailand. By using this screening strategy, 9 fungal strains were selected based on their potential for cellulase production. Based on sequence-based identification of these fungal isolates, 4 species in 4 genera were identified: Aspergillus terreus (3 strains: AG466, AG438 and AG499), Penicillium oxalicum (4 strains: AG452, AG496, AG498 and AG559), Talaromyces siamensis (1 strain: AG548) and Trichoderma afroharzianum (1 strain: AG500). After examining their lignocellulose degradation capacity, our data showed that P. oxalicum AG452 exhibited the highest glucose yield after saccharification of pretreated sugarcane trash, cassava pulp and coffee silver skin. In addition, Ta. siamensis AG548 produced the highest glucose yield after hydrolysis of pretreated sugarcane bagasse. Our study demonstrated that the proposed two-step screening strategy can be further applied for discovering cellulolytic fungal potentials isolated from various environmental samples. The fungal strains isolated in this study can be further applied for bioconversion of agricultural lignocellulosic residues into valuable biotechnological products.

PMID: 32876068 [PubMed – as supplied by publisher]

Source: Industry