Determination of Mold Contamination Using Ergosterol Imprinted Particles.
Biotechnol Prog. 2020 Oct 05;:e3089
Authors: Oktay Başeğmez Hİ, Baydemir Peşint G, Nergiz M, Zenger O
Ergosterol is a key biochemical marker for fungal mycelial growth. In this study, molecularly ergosterol imprinted particles (Erg-MIPs) were newly synthesized for the selective detection of Ergosterol in mold samples. Erg-MIPs were characterized via scanning electron microscopy (SEM), swelling studies and surface area measurements. Maximum selective Ergosterol adsorption achieved as 28.50 mg/g Erg-MIP. Selectivity studies showed that Erg-MIPs adsorbed Erg 2.01 and 3.27 times higher than that of cholesterol and stigmasterol respectively. Erg adsorption from Aspergillus niger was found as 23.87 mg/g. Reusability of Erg-MIPs was studied and decrease in Erg adsorption capacity of the particles was negligible (3%). Erg-MIPs are good affinity materials for the selective Erg detection from food samples, prior to use in food industry. This article is protected by copyright. All rights reserved.
PMID: 33016620 [PubMed – as supplied by publisher]