Medical and cytotoxicity effects of green synthesized silver nanoparticles using Achillea millefolium extract on MOLT-4 lymphoblastic leukemia cell line.

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Medical and cytotoxicity effects of green synthesized silver nanoparticles using Achillea millefolium extract on MOLT-4 lymphoblastic leukemia cell line.

J Med Virol. 2020 Nov 25;:

Authors: Karimi S, Shahri MM

Abstract
Green Chemistry which aims at development of efficient methods for the synthesis of nanoparticles is a relatively new emerging field of nanotechnology which has economic and environmental friendly benefits over chemical and physical processes. The present work was carried out to develop silver nanoparticles (Ag-NPs) using the plant (Achillea millefolium or yarrow) aqueous extract as both reducing and capping agent under green synthesis method. Characterization of synthesized Ag-NPs was done using IR Spectroscopy, Scanning Electron Microscopy (SEM), X-ray diffraction (XRD) and Ultraviolet-visible (UV-vis). UV-Vis spectrum showed maximum absorbance at around 440-470 nm which suggested the formation of green synthesized Ag-NPs. The morphological study demonstrated that the Ag-NPs were spherical in shape with the average size of 22.4 ± 7.4 nm. The antimicrobial activities of Ag-NPs against Fusarium and Aspergillus niger species of fungal and Escherichia coli species of bacteria was investigated through disc diffusion and well diffusion method using their zone of inhibition. The cytotoxicity effect of Ag-NPs on cell lines MOLT-4 was evaluated by using MTT assay. These nanoparticles showed significant antimicrobial activity on samples, so that they were prevented the growth of bacteria and fungus in very low concentration. In vitro cytotoxicity studies on MOLT-4 cells shows that IC50 of green synthesized Ag-NPs was 0.011 µm in comparison to 1.7 for Cisplatin which more active than anticancer drug for MOLT-4 cell line. The results showed that the green synthesized Ag-NPs are expected to have notable applications and can potentially useful in pharmaceutical and biomedical applications. This article is protected by copyright. All rights reserved.

PMID: 33236797 [PubMed – as supplied by publisher]

Source: Industry