Effective treatment of cutaneous mold infections by antimicrobial blue light that is potentiated by quinine.

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Effective treatment of cutaneous mold infections by antimicrobial blue light that is potentiated by quinine.

J Infect Dis. 2021 Feb 02;:

Authors: Leanse LG, Dos Anjos C, Wang Y, Murray CK, Hooper DC, Dai T

Abstract
BACKGROUND: Cutaneous mold infections commonly result from an array of traumatic injuries that involve direct inoculation of contaminated soil into wounds. Here, we explored the use of antimicrobial blue light (aBL; 405 nm wavelength) and the combination of aBL with quinine hydrochloride (aBL + Q-HCL) for the treatment of cutaneous mold infections.
METHODS: Efficacy of aBL and aBL + Q-HCL in killing clinically important pathogenic molds (Aspergillus fumigatus, Aspergillus flavus, and Fusarium oxyprorum) was investigated. Ultra-performance liquid chromatography (UPLC) identified and quantified endogenous porphyrins in the mold conidia. Finally, a mouse model of dermabrasion wound infected with a bioluminescent variant of A. fumigatus was developed to investigate the efficacy of aBL in treating cutaneous mold infections.
RESULTS: We demonstrated that mold conidia are tolerant to aBL, but Q-HCL enhances efficacy. Transmission electron microscopy revealed intracellular damage by aBL. aBL + Q-HCL resulted in intracellular and cell wall damage. Porphyrins were observed in all mold strains, with A. fumigatus having the highest concentration. aBL and aBL + Q-HCL effectively reduced the burden of A. fumigatus within an established dermabrasion infection that limited recurrence post-treatment.
CONCLUSIONS: aBL and aBL + Q-HCL may offer a novel approach for the treatment of mold infections.

PMID: 33528496 [PubMed – as supplied by publisher]

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