Enhanced enzymatic performance of immobilized lipase on metal organic frameworks with superhydrophobic coating for biodiesel production
J Colloid Interface Sci. 2021 Jun 10;602:426-436. doi: 10.1016/j.jcis.2021.06.017. Online ahead of print.
Inspired by the interfacial catalysis of lipase, Herein, the hydrophobic ZIF-L coated with polydimethylsiloxane (PDMS) were prepared by chemical vapor deposition (CVD) and used to immobilize lipase from Aspergillus oryzae (AOL) for biodiesel production. The results showed that the PDMS coating enhanced the stability of ZIF-8 and ZIF-L in PBS. Immobilization efficiency of AOL on PDMS-modified ZIF-L was 96% under optimized conditions. The resultant immobilized lipase (AOL@PDMS-ZIF-L) exhibited higher activity recovery (430%) than AOL@ZIF-L. Meanwhile, compared with free lipase, the AOL@PDMS-ZIF-L exhibited better storage stability and thermal stability. After 150 days of storage, the free lipase retained only 20% of its original activity of hydrolyzing p-NPP, while the AOL@PDMS-ZIF-L still retained 90% of its original activity. The biodiesel yield catalyzed from soybean oil by free lipase was only 69%, However, the biodiesel yield by AOL@PDMS-ZIF-L reached 94%, and could still be maintained at 85% even after 5 consecutive cycles. It is believed that this convenient and versatile strategy has great promise in the important fields of immobilized lipase on MOF for biodiesel production.