Purification, structural elucidation and anticancer activity of a compound extracted from Aspergillus fumigatus strain MF-1

Arch Microbiol. 2021 Jun 24. doi: 10.1007/s00203-021-02453-w. Online ahead of print.

ABSTRACT

The fungus is a great source of secondary metabolites which may possess various biological activities for the treatment of human diseases and disorders. The present study aimed to identify the secondary metabolites from Aspergillus fumigatus strain MF-1 using analytical techniques (chromatographic and spectroscopic). The instruments includes ¹H-NMR, ¹³C-NMR, DEPT, COSY, HMQC, FTIR and UV-Vis spectrophotometer were employed to study the extracted compound and the produced compound is 2,5-dioxocyclopentylamino-7-oxohepta-1,3,5-trienyl-2,5-dihydroxy-3-chlorophenyl-2,4,6-trimethyldeca-2,4-dienamide. Furthermore, the four fractions were tested over HeLa cell lines for their cytotoxicity performance and found that fraction 4 exhibited potent cytotoxic effect with IC₅₀ value of 74.38 ± 0.31 µg/mL on HeLa cell lines than other fractions.

PMID:34169338 | DOI:10.1007/s00203-021-02453-w