Evaluation of the biological activities of β-glucan isolated from Lentinula edodes

Lett Appl Microbiol. 2022 Apr 28. doi: 10.1111/lam.13727. Online ahead of print.

ABSTRACT

Lentinula edodes (shiitake) is an edible mushroom that contains many constituents and β-glucan is considered a major polysaccharide. This study, therefore, aimed to characterize β-glucan and evaluate its activities. Fresh fruit bodies of L. edodes were used for β-glucan extraction and followed by analyses via Fourier transform infrared, Nuclear magnetic resonance, and High Performance Liquid Chromatography confirming its characteristic structure. For evaluating biological activities of β-glucan, different concentrations (0.1-3.5 mg ml-1 ) were assessed. The optimal dose was 3.5 mg ml-1 that showed the highest scavenging radical ability (75.3%) confirms antioxidant activity, strong inhibition of the peroxyl radical (80.9%) to inhibit lipid peroxidation, elevation the inhibition percentage of both α-amylase (73.4%) and α-glucosidase (70.3%) indicates the antidiabetic properties, and highest AFB1 reduction (88%) which ensured the aflatoxin-detoxifying ability. In addition, antifungal activity of β-glucan was evaluated to inhibit sporulation process in Aspergillus niger and recorded with minimum inhibitory concentration of 2.5 mg ml-1 and minimum fungicidal concentration of 3 mg ml-1 . In a dose-dependent manner, higher concentration of β-glucan affects viability of tumor cells concomitant induces potent anti-cancer immune responses and inhibited the activity of topoisomerase I which are considered an important target for cancer chemotherapy. Therefore, L. edodes-β-glucan has the potential to act as a suggestive agent for antioxidant, antidiabetic, antifungal activity, and aflatoxin detoxification.

PMID:35482469 | DOI:10.1111/lam.13727

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