Application of Principal Component Analysis (PCA) to the Evaluation and Screening of Multiactivity Fungi

J Ocean Univ China. 2022;21(3):763-772. doi: 10.1007/s11802-022-5096-x. Epub 2022 May 13.


Continued innovation in screening methodologies remains important for the discovery of high-quality multiactive fungi, which have been of great significance to the development of new drugs. Mangrove-derived fungi, which are well recognized as prolific sources of natural products, are worth sustained attention and further study. In this study, 118 fungi, which mainly included Aspergillus spp. (34.62%) and Penicillium spp. (15.38%), were isolated from the mangrove ecosystem of the Maowei Sea, and 83.1% of the cultured fungi showed at least one bioactivity in four antibacterial and three antioxidant assays. To accurately evaluate the fungal bioactivities, the fungi with multiple bioactivities were successfully evaluated and screened by principal component analysis (PCA), and this analysis provided a dataset for comparing and selecting multibioactive fungi. Among the 118 mangrove-derived fungi tested in this study, Aspergillus spp. showed the best comprehensive activity. Fungi such as A. clavatonanicus, A. flavipes and A. citrinoterreus, which exhibited high comprehensive bioactivity as determined by the PCA, have great potential in the exploitation of natural products and the development of new drugs. This study demonstrated the first use of PCA as a time-saving, scientific method with a strong ability to evaluate and screen multiactive fungi, which indicated that this method can affect the discovery and development of new drugs.

PMID:35582545 | PMC:PMC9098371 | DOI:10.1007/s11802-022-5096-x

Source: Industry