Identification of a D-galacturonate reductase efficiently using NADH as a cofactor

Biotechnol Rep (Amst). 2022 Jun 2;35:e00744. doi: 10.1016/j.btre.2022.e00744. eCollection 2022 Sep.

ABSTRACT

D-galacturonate reductases are catalysing the reversible reduction of D-galacturonate to L-galactonate using NAD(P)H as a cofactor. The enzymes are part of two different pathways. One pathway is the fungal pathway for the catabolism of the main compound of pectin, D-galacturonate. The other pathway is a a pathway in plants for L-ascorbic acid synthesis. The previously described naturally occurring enzymes preferably use NADPH as a cofactor. Although certain D-galacturonate reductases, such as the reductases from Aspergillus niger or Euglena gracilis also accept NADH, their activity is significantly higher with NADPH. We identified in E. gracilis a gene, called gaa1, coding for a D-galacturonate reductase with similar activities with NADH and NADPH. It is potentially useful for the metabolic engineering of microbes to make use of pectin rich biomass.

PMID:35711324 | PMC:PMC9192788 | DOI:10.1016/j.btre.2022.e00744

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