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Real-Time PCR kit for the detection of Pneumocystis jirovecii


  • Pneumocystis jirovecii
  • Human β-globin gene (Endogenous Internal Control)
  • Internal Extraction Control (IEC)

Product Code: OLM2008


50 reactions


Features and benefits

  • Aids in the diagnosis of Pneumocystis Pneumonia
  • Human β-globin gene detection is included as an endogenous internal control for the assessment of specimen quality and nucleic acid extraction efficiency
  • Culture-independent diagnostics
  • Results within 45 minutes of nucleic acid extraction
  • ‘Ready to use’ reagents – no resuspension/dilution steps required
  • Compatible with existing laboratory equipment
  • Enabling quick laboratory reporting to support clinical decision-making when time is of the essence

Diagnostic specimens

  • Bronchoalveolar lavage (BAL) samples
  • Respiratory Wash

Quality Assured

  • Validated on clinical samples
  • Internal Extraction Control (IEC) included
  • Positive Control (PC) included
  • Endogenous Control detection for assessment of specimen quality
  • Validated on QCMD EQA programmes
  • CE-IVD marked

Performance Characteristics

  • Sensitive to <1 Pneumocystis genome copy
  • Proven efficacy in the diagnosis of PCP

Kit Contents

  • Primer/Probe Mix
  • qPCR Master Mix
  • RNase/DNase-free water
  • Positive Control
  • Internal Extraction Control (IEC)


Pneumocystis jirovecii pneumonia (PCP) is a life-threatening opportunistic disease caused by Pneumocystis jirovecii, formerly known as Pneumocystis carinii species, a unicellular eukaryotic and ubiquitous yeast-like fungus. Conservative approximations calculate around 400,000 cases of PCP annually worldwide, but this is likely to be an underestimate of the true burden. It is estimated that up to 20% of adults might carry this fungus at any given time, without any symptoms, resulting in continuous interindividual circulation responsible for transmission to immunocompromised individuals who are at high risk of developing PCP. Amongst them, around 30-40% of PCP infections occur in individuals with human immunodeficiency virus (HIV) infection/acquired immune deficiency syndrome (AIDS). In addition to HIV coinfection, other risk factors include chronic lung diseases, cancer, inflammatory or autoimmune diseases and solid organ or stem cell transplant. Once PCP is clinically suspected, the diagnosis should be time-sensitive, and treatment should be initiated as soon as possible to avoid further complications.

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